D8764
Deoxyribonuclease II from bovine spleen
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
Manufacturer: Sigma Aldrich
CAS Number: 9025-64-3
Synonym(S): DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 30000 UNITS | D8764-30000-UNITS | In Stock | ₹ 16,749.90 |
| 150000 UNITS | D8764-150000-UNITS | In Stock | ₹ 54,112.50 |
| 300000 UNITS | D8764-300000-UNITS | In Stock | ₹ 83,716.20 |
D8764 - 30000 UNITS
In Stock
Quantity
1
Base Price: ₹ 16,749.90
GST (18%): ₹ 3,014.982
Total Price: ₹ 19,764.882
biological source
bovine spleen
Quality Level
200
type
Type V
form
essentially salt-free, lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
38 kDa
concentration
≥50% protein
technique(s)
DNA extraction: suitabletissue culture: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
Other Options
Related Products
Description
- Application: Deoxyribonuclease II from bovine spleen has been used: as a component of digestion mix to isolate immune cells from colon samples[1]to digest retinal explants for single-cell suspension preparation[2]for isolation of stromal cells from human colon tissue[3]in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart[4]
- Biochem/physiol Actions: Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.[5]
- Unit Definition: One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable