N5661
Nuclease S1 from Aspergillus oryzae
for single-strand DNA/RNA digestion
Manufacturer: Sigma Aldrich
CAS Number: 37288-25-8
Synonym(S): Endonuclease S1
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50000 UNITS | N5661-50000-UNITS | In Stock | ₹ 30,846.90 |
N5661 - 50000 UNITS
In Stock
Quantity
1
Base Price: ₹ 30,846.90
GST (18%): ₹ 5,552.442
Total Price: ₹ 36,399.342
biological source
Aspergillus sp. (A. oryzae)
Quality Level
200
form
solution
concentration
≥100000 units/mL
technique(s)
DNA purification: suitable
suitability
suitable for nucleic acid purification
application(s)
cell analysis
shipped in
wet ice
storage temp.
−20°C
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | 37288-25-8 | MUNG BEAN NUCLEASE | -- | -- |
Related Products
Description
- General description: The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides. [1]
- Application: Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. [2] It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor. [3]
- Biochem/physiol Actions: Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.
- Unit Definition: One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.
- Physical form: Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml protein
SAFETY INFORMATION
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable