P0114
Phosphatase, Alkaline from bovine intestinal mucosa
BioUltra, ≥5,700 DEA units/mg protein
Manufacturer: Sigma Aldrich
CAS Number: 9001-78-9
Synonym(S): Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10000 UNITS | P0114-10000-UNITS | In Stock | ₹ 58,641.30 |
| 50000 UNITS | P0114-50000-UNITS | In Stock | ₹ 2,18,192.70 |
P0114 - 10000 UNITS
In Stock
Quantity
1
Base Price: ₹ 58,641.30
GST (18%): ₹ 10,555.434
Total Price: ₹ 69,196.734
product line
BioUltra
Quality Level
200
form
(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
specific activity
≥5,700 DEA units/mg protein
mol wt
dimer ~160 kDa
concentration
≥10 mg/mL
shipped in
wet ice
storage temp.
2-8°C
Other Options
Related Products
Description
- General description: Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. [1]
- Application: Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
- Biochem/physiol Actions: Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A. [4][5]
- Physical properties: Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
- Unit Definition: One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)
- Physical form: Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6
- Analysis Note: Package sizes are based on DEA units
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves