Description

• Application: Pronase E can be used to degrade antheraea pernyi silk fibroin films. [1]
• Biochem/physiol Actions: Alkaline protease that is approximately twice as active at pH 11.0 than at the usual assay conditions for protease, pH 7.5 and 37 °C. By comparison, P5147 Type XIV protease is only approximately 25% as active at pH 11.0, 30 °C.
• Physical properties: Isoelectric point : 8.7Inhibitors : Diisopropyl fluorophosphate, EDTAOptimum pH : >=12 Optimum temperature : 60oCpH Stability : pH 5.0 - 11.5 (25oC,24hr) Thermal stability : below 50oC (pH 8.3, 15min)
• Unit Definition: One unit will hydrolyze casein to produce peptide equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 11.0 at 30 °C.
• Physical form: This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
• Preparation Note: Collected from culture broth of S.Sp