P1512
Thermolysin from Geobacillus stearothermophilus
Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)
Manufacturer: Sigma Aldrich
CAS Number: 9073-78-3
Synonym(S): Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 MG | P1512-25-MG | In Stock | ₹ 5,616.60 |
| 100 MG | P1512-100-MG | In Stock | ₹ 13,974.90 |
| 250 MG | P1512-250-MG | In Stock | ₹ 26,751.00 |
| 1 G | P1512-1-G | In Stock | ₹ 85,481.10 |
P1512 - 25 MG
In Stock
Quantity
1
Base Price: ₹ 5,616.60
GST (18%): ₹ 1,010.988
Total Price: ₹ 6,627.588
biological source
Geobacillus stearothermophilus
Quality Level
300
type
Type X
form
lyophilized powder
specific activity
30-350 units/mg protein (E1%/280)
mol wt
34.6 kDa by amino acid sequence
purified by
crystallization
shipped in
wet ice
storage temp.
−20°C
Other Options
Related Products
Description
- General description: Thermolysin is a protease that has specificity different from other proteases available for sequence investigations. [1]
- Application: Non-specific Protease Activity Assay Video
- Quality: Contains many extraneous enzymes.
- Unit Definition: One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
- Physical form: lyophilized powder containing calcium and sodium acetate buffer salts
- Preparation Note: The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves