P5931
Phosphatase, Alkaline from Escherichia coli
lyophilized powder, 30-60 units/mg protein (in glycine buffer)
Manufacturer: Sigma Aldrich
CAS Number: 9001-78-9
Synonym(S): Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 UNITS | P5931-100-UNITS | In Stock | ₹ 27,217.20 |
| 200 UNITS | P5931-200-UNITS | In Stock | ₹ 45,854.10 |
| 500 UNITS | P5931-500-UNITS | In Stock | ₹ 1,00,455.00 |
P5931 - 100 UNITS
In Stock
Quantity
1
Base Price: ₹ 27,217.20
GST (18%): ₹ 4,899.096
Total Price: ₹ 32,116.296
form
lyophilized powder
Quality Level
200
specific activity
30-60 units/mg protein (in glycine buffer)
composition
Protein, ~50% biuret
storage temp.
−20°C
Other Options
Related Products
Description
- General description: Alkaline phosphatase (ALP) of E.coli is a member of an enzyme group that possesses intragenic complementation.[1]
- Application: Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P5931 has been used during immunoblots to treat cell membranes prior to Tau1 incubation[2].
- Biochem/physiol Actions: Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium[4][5][6]. E. coli akaline phosphatase has a broad specificity for phosphate esters[4].
- Unit Definition: One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
- Physical form: Lyophilized powder containing Tris buffer salts, MgSO4, and ZnSO4
- Preparation Note: Chromatographically purified
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves