P6774
Phosphatase, Alkaline from bovine intestinal mucosa
buffered aqueous solution, ≥2,000 DEA units/mg protein
Manufacturer: Sigma Aldrich
CAS Number: 9001-78-9
Synonym(S): Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1000 UNITS | P6774-1000-UNITS | In Stock | ₹ 9,823.50 |
| 2000 UNITS | P6774-2000-UNITS | In Stock | ₹ 14,751.90 |
| 10000 UNITS | P6774-10000-UNITS | In Stock | ₹ 44,411.10 |
| 50000 UNITS | P6774-50000-UNITS | In Stock | ₹ 1,78,132.80 |
P6774 - 1000 UNITS
In Stock
Quantity
1
Base Price: ₹ 9,823.50
GST (18%): ₹ 1,768.23
Total Price: ₹ 11,591.73
biological source
bovine intestinal mucosa
Quality Level
200
form
buffered aqueous solution
specific activity
≥2,000 DEA units/mg protein
mol wt
dimer ~160 kDa
concentration
5-20 mg/mL
storage temp.
2-8°C
Other Options
Related Products
Description
- Application: Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
- Biochem/physiol Actions: Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.
- Physical properties: Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
- Unit Definition: One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)
- Physical form: Solution in 3.0 M NaCl containing 5 mM MgCl2, 0.2 mM ZnCl2, and 30 mM triethanolamine, pH 7.6
- Preparation Note: Affinity purified
- Analysis Note: Package sizes are based on DEA units
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves