P6912
o-Phenylenediamine dihydrochloride
peroxidase substrate, chromogenic, tablet
Manufacturer: Sigma Aldrich
CAS Number: 615-28-1
Synonym(S): 1,2-Phenylenediamine tablet, OPD, OPD Tablet
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 TABS | P6912-50-TABS | In Stock | ₹ 17,404.80 |
| 100 TABS | P6912-100-TABS | In Stock | ₹ 29,670.30 |
P6912 - 50 TABS
In Stock
Quantity
1
Base Price: ₹ 17,404.80
GST (18%): ₹ 3,132.864
Total Price: ₹ 20,537.664
product name
o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet
Quality Level
300
form
tablet
mp
258 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
Other Options
Related Products
Description
- General description: o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
- Application: o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in ELISA (enzyme-linked immunosorbent assay).[1][2][3]
- Packaging: 150 mg total tablet weight
- Reconstitution: Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
SAFETY INFORMATION
Pictograms
Signal Word
Warning
Hazard Statements
Precautionary Statements
P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
Hazard Classifications
Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable