P8361
Phosphatase, Alkaline bovine
recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein
Manufacturer: Sigma Aldrich
CAS Number: 9001-78-9
Synonym(S): Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5000 UNITS | P8361-5000-UNITS | In Stock | ₹ 53,180.10 |
P8361 - 5000 UNITS
In Stock
Quantity
1
Base Price: ₹ 53,180.10
GST (18%): ₹ 9,572.418
Total Price: ₹ 62,752.518
recombinant
expressed in Pichia pastoris
Quality Level
200
form
solid
specific activity
≥4000 units/mg protein
mol wt
apparent mol wt 160 kDa
storage temp.
2-8°C
Other Options
Related Products
Description
- Application: Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase. Product P8361 has been used to dephosphorylate Dishevelled (Dvl) , which is a key component in the Wnt/β-catenin signaling pathway[1].
- Biochem/physiol Actions: Alkaline phosphatase dephosphorylates proteins and nucleic acids.
- Unit Definition: One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves