P8375
Peroxidase from horseradish
Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)
Manufacturer: Sigma Aldrich
CAS Number: 9003-99-0
Synonym(S): Detection Enzyme, HRP, Peroxidase, Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1000 UNITS | P8375-1000-UNITS | In Stock | ₹ 7,292.70 |
| 2000 UNITS | P8375-2000-UNITS | In Stock | ₹ 12,087.90 |
| 5000 UNITS | P8375-5000-UNITS | In Stock | ₹ 12,554.10 |
| 10000 UNITS | P8375-10000-UNITS | In Stock | ₹ 27,017.40 |
| 25000 UNITS | P8375-25000-UNITS | In Stock | ₹ 46,164.90 |
| 100000 UNITS | P8375-100000-UNITS | In Stock | ₹ 1,99,278.30 |
| 250000 UNITS | P8375-250000-UNITS | In Stock | ₹ 3,42,723.60 |
P8375 - 1000 UNITS
In Stock
Quantity
1
Base Price: ₹ 7,292.70
GST (18%): ₹ 1,312.686
Total Price: ₹ 8,605.386
type
Type VI
Quality Level
300
form
essentially salt-free, lyophilized powder
specific activity
≥250 units/mg solid (using pyrogallol)
mol wt
~44 kDa
solubility
0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)
absorbance ratio
RZ 2.5-4.0
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
InChI
1S/H2O3/c1-3-2/h1-2H
Other Options
Related Products
Description
- General description: Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
- Application: Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases.. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8375, type VI, is an essentially salt free lyophilized powder. It is commonly used to determine amounts of glucose and peroxides in solution. It has been used to study sensory input of cervical spinal cord neurons[1].
- Biochem/physiol Actions: HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase and hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.[4]
- Unit Definition: One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
- Analysis Note: The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
- Other Notes: View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves