4532026
Thermo Scientific™ Pierce™ Recombinant Protein G
Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.
Manufacturer: Fischer Scientific
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Product Line
Pierce™
Protein Subtype
Protein G
Content And Storage
Upon receipt store at -20°C. Product is shipped at ambient temperature.
Form
Powder
Target
Antibodies
Related Products
Description
- Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols
- Protein G is a bacterial cell wall protein isolated from group G Streptococci
- DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding
- The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples
- Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2
- Highlights: Contains two Fc-binding domains per protein Better than Protein A for mouse (incl
- IgG1), human (incl
- IgG3) and rat, goat and cow antibodies Poorer than Protein A for guinea pig, pig, dog and cat Does not bind human IgM, IgD or IgA