Thermo Scientific™ Pierce™ Recombinant Protein A

Description

• Use Thermo Scientific Pierce Recombinant Protein A to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols
• Features of Recombinant Protein A include: Contains four Fc-binding domains per protein Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate
• Recombinant Protein A is produced in E
• coli and functions essentially the same as native Protein A
• The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit
• Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6)
• Properties of Recombinant Protein A Source: E
• coli Molecular Weight: ∼44,600 (Apparent MW by SDS-PAGE: 45,000) Form: salt-free powder A280 of 0.1% solution: ∼0.165 Isoelectric point (pI): 4.7–4.8 The interaction between Protein A and IgG is not equivalent for all species
• Even within a species, Protein A interacts with some subclasses of IgG and not others
• For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind
• There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.