Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Description

• Thermo Scientific™ Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed
• Performing gel electrophoresis and duplicate immunoblot assays to test new primary antibodies or antibody concentrations is time-consuming and expensive
• Restore Western Blot Stripping Buffer eliminates this waste when detecting immunoblots with chemiluminescent Western blotting substrates
• The reagent allows clean and efficient removal of primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen
• This allows blots to be stripped and reprobed with confidence
• Highlights: Saves time – no need to re-run gels and blots Saves costly sample – re-probe the membrane using the same target sample Effective – formulation is more efficient at stripping antibodies than homemade buffers Gentle – does not damage the target antigen during stripping allowing efficient reprobing Odor-free – no mercaptans means no acrid odors Economical – less expensive than other commercial stripping buffers Reuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody; Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time.