Invitrogen™ No-Stain™ Protein Labeling Reagent
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A44717-Each-of-1 | In Stock | ₹ 4,876.92 |
A44717 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,876.92
GST (18%): ₹ 877.846
Total Price: ₹ 5,754.766
Detection Method
Fluorescence
Product Line
Invitrogen™
Gel Compatibility
Mini Precast Protein Gels, Midi Precast Protein Gels
Shipping Condition
Dry Ice
For Use With (Equipment)
Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Run Time
10 minutes
Content And Storage
• No-Stain activator, 200 μL, store at -5°C to -30°C• No-Stain derivatizer, 200 μL, store at -5°C to -30°C• No-Stain labeling buffer (20X), 2 x 5 mL, store at 15°C to 30°C
Membrane Compatibility
PVDF, Nitrocellulose
No. of Reactions
A standard kit will label 10 mini gels or 10 mini gel-sized membranes
Description
The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type
Protein Labeling Reagent
For Use With (Application)
Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Related Products
Description
- Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer
- Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer
- Easy to use protocol—Mix and incubate with the transferred membrane
- The reaction time is 10 minutes
- Gel staining is also a 10 minute reaction
- Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image
- Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well
- Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection
- Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.
SAFETY INFORMATION
- ShelfLife : At least 2 years from receipt