Thermo Scientific™ HisProbe™-HRP Conjugate

Description

• Thermo Scientific™ HisProbe-HRP is an nickel (Ni 2 +)-activated derivative of horseradish peroxidase (HRP)
• This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins
• Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags
• The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate
• The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules
• The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC)
• Highlights: Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies