Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Description

• Thermo Scientific™ Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes
• The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody
• The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL
• The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods
• Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed
• Highlights: Fast – all the sensitivity of Pierce ECL substrate; saves three to four hours over traditional blotting methods Convenient – no expensive hardware or extra bench space needed; eliminates membrane clogging issues Economical – costs about the same as a classical Western blot; no expensive consumables or extra equipment Easy – optimized kits includes all reagents except primary antibody Versatile – kits work with both rabbit and mouse primary antibodies Includes: Kits contain all reagents to perform Western blots probed with mouse and rabbit primary antibodies, including: Fast Western Antibody Diluent; Fast Western 10X Wash Buffer; Fast Western Optimized HRP Reagent; Pierce ECL Detection Reagent 1; Pierce ECL Detection Reagent 2