Invitrogen™ iBlot™ 2 Gel Transfer Device

Description

• The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)
• Complete protein transfer in 7 minutes or less High detection sensitivity and even transfer Increased blotting reliability and reproducibility Flexible gel size formats and membrane types A simple, user-friendly system Options to create new custom programs Built-in tutorial and application notes High-quality transfer stacks that are more compact than before Rapid, High-quality Protein Transfers For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency Allows users to create custom programs iBlot 2 Transfers Save Time With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques How It Works Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers The high density of ions in the gel matrix enables rapid protein transfer During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen) Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer Proteins, Expression, Isolation and Analysis, Western Blotting