Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution

Description

• Thermo Scientific Pierce 1-Step Chloronaphthol Solution is a solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods
• Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples
• The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB
• However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications
• Features of Chloronaphthol Substrates: HRP substrate —for detection of horseradish peroxidase activity on solid media Chromogenic —no special equipment needed for visualization Blue-purple —easy to photograph in color or black-and-white Package options —choose powder or ready-to-use solution Highlights of Pierce CN Solution Ready-to-use —no ethanol or methanol required to dissolve; no dilution necessary for use Complete —does not require buffer or addition of hydrogen peroxide Pre-filtered —does not need to be filtered before use like powders or tablets after they are dissolved Stable —store refrigerated at 4°C for at least one year