Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 570, eBioscience™, Invitrogen™

Description

• Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin
• Applications Reported: This eBMG2b antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry
• Applications Tested: This eBMG2b antibody has been tested by immunocytochemistry on fixed and permeabilized cells
• Use isotype control at same concentration as experimental antibody
• This product has not been validated for flow cytometric analysis
• Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70)
• A standard Alexa Fluor® 555 or TRITC filter is acceptable
• Excitation: 555 nm; Emission: 570 nm Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.