Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin
• Applications Reported: PerCP-eFluor® 710 Rat IgG1 Isotype Control has been reported for use in flow cytometric analysis
• Applications Tested: PerCP-eFluor® 710 Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells
• Isotype control should be used at the same concentration as experimental antibody
• PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
• PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative
• Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols
• Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics
• These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants
• Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens
• It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens
• Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases
• With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.