Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin
• Applications Reported: APC-eFluor™ 780 Rat IgG1 Isotype Control (APC-Alexa Fluor™ 750 replacement) has been reported for use in flow cytometric analysis
• Applications Tested: Rat IgG1 K Isotype Control APC-eFluor™ 780 has been tested by flow cytometric analysis of mouse splenocytes
• Use isotype control at the same concentration as experimental antibody
• APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Light sensitivity: This tandem is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics
• These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants
• Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens
• It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens
• Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases
• With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.