Syrian Hamster IgG Isotype Control, PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum
• Applications Reported: This isotype control antibody has been reported for use in flow cytometric analysis
• Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells
• It should be used at the same concentration as the experimental antibody
• PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
• PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative
• Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols
• Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.