Syrian Hamster IgG Isotype Control, Alexa Fluor™ 532, eBioscience™, Invitrogen™

Description

• Description: This polyclonal hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum
• Applications Reported: This isotype control antibody has been reported for use in flow cytometric analysis
• Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes and should be used at the same concentration as the experimental antibody
• Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm
• This cannot be used with the Yellow-Green laser (561 nm)
• We recommend using a 560/14 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.