Dinitrophenyl-KLH Polyclonal Antibody, Alexa Fluor™ 488, Invitrogen™

Description

• The anti-DNP-KLH antibody is supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, containing 5 mM sodium azide
• The degree of labeling is indicated on the product label
• This antibody was raised in rabbit against DNP conjugated to keyhole limpet hemocyanin
• The IgG fraction, which contains both anti-DNP and anti-KLH IgGs, was isolated from total serum and determined to be >95% pure by SDS-polyacrylamide gel electrophoresis
• It is a good practice to centrifuge protein conjugate solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment
• This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining
• Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically
• Peak absorption and fluorescence emission wavelengths for Alexa Fluor™ 488 anti-DNPKLH conjugate is 494 nm and 520 nm, respectively Because of their high affinity for the dinitrophenyl hapten1,2 (DNP), Invitrogen's antibodies against the dinitrophenyl- keyhole limpet hemocyanin conjugate (anti-DNP-KLH) are sensitive probes for detecting DNP-labeled molecules
• The fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes
• Dinitrophenyl-KLH Polyclonal antibody specifically detects Dinitrophenyl-KLH in Chemical samples
• It is validated for Immunocytochemistry, Immunohistochemistry, Western Blot.