dsRNA Antibody Pack, Novus Biologicals™

dsRNA

Monoclonal

ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Dot Blot, Immunoaffinity Purification

Double-stranded RNA

Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clones.

3 Vials

Primary

The lyophilised samples should each be reconstituted with 100 ul sterile distilled water.

Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.

ELISA, Immunohistochemistry, Immunofluorescence, Dot Blot, Affinity Purification

Unconjugated

After reconstitution the mAbs will be in PBS without any stabilisers or preservatives at a concentration of 1 mg/ml

Mouse

Protein A purified

RUO

Mouse monoclonal antibodies NBP3-11395, NBP3-11396, and NBP3-11397 recognize double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognized by all three antibodies, although in some assays the affinity of NBP3-11395 and NBP3-11396 to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens. NBP3-11397 shows higher affinity to poly(I).poly(C) than to the other dsRNA antigens, although the difference of apparent binding constants may vary under different experimental conditions.

Multi-species

Purified