Invitrogen™ NA-Fluor™ Influenza Neuraminidase Assay Kit

Description

• Efficient design - neuraminidase assay in one complete kit Optimized Formulations - assay reagents have been optimized for maximum performance based on NISN protocols Robust Application - functional detection of NI-resistant virus in mixed viral populations Easy of use and Flexible Screening - stable fluorescent signal enables batch-mode or high throughput processing Specifications Color: Blue Detection Method: Fluorescent Filter Type: Emission 440-460, Excitation 350-365 For Use With Equipment: Fluorometer Form: Lyophilized Format: 96-well plate High Throughput Compatibility: High Throughput-Compatible Neuraminidase Assay in One Complete Kit The NA-Fluor Influenza Neuraminidase Assay Kit includes comprehensive protocols for titering viral isolates based upon neuraminidase activity and conducting neuraminidase enzyme inhibition assays
• The assay can also be used for monitoring neuraminidase enzyme activity from non-viral or bacterial sources
• The NA-Fluor Kit contains reagents for ten 96-well microplates sufficient for a total of 960 assay wells and includes the following components: fluorescent MUNANA neuraminidase substrate, assay buffer, and stop solution to enhance and stabilize signal
• The assay is performed in standard black microplates (microplates not provided in kit) and is read on standard fluorometers
• Neuraminidase Assay Compares to NISN Protocols The NA-Fluor assay reagent formulations are optimized to be comparable to several well-established MUNANA-based assay protocols, such as: The MUNANA substrate concentration, assay buffer formulation and assay conditions are consistent with NISN IC-50 determination protocols Data generated using the NA-Fluor assay corresponds to data generated with established MUNANA based protocols These key parameters enable investigators to compare data acquired during current NI resistance surveillance screens using the NA-Fluo™ assay to data acquired using their previous MUNANA assay protocols Detection of NI-Resistant Virus in Mixed Viral Populations The large shift in IC-50 values between sensitive and oseltamivir-resistant virus using the NA-Fluor assay enables detection of mutant virus in mixed viral samples
• This capability is critical for identifying resistant virus in clinical isolates presenting mixed populations of resistant and sensitive virus during NI susceptibility surveillance
• Flexibility for Screening Neuraminidase Activity The NA-Fluor assay provides an easy, flexible format for the screening of several to several hundred viral isolates, or the screening of thousands of compounds during high-throughput lead discovery with quality data at high confidence levels
• With superior performance, the assay has demonstrated a Z' of 0.78 to 0.8, making it strongly capable for use in high-throughput screening
• The fluorescent reaction product remains stable for hours at room temperature after the assay is complete, enabling read-time flexibility and comparable data from first plate to last
• The assay signal remains nearly constant and IC-50 values are identical from data collected up to 4 hours at room temperature and up to 4 days at 4°C after assay termination
• The NA-Fluor assay has been optimized as an end-point assay run at 37°C for one hour following NI pre-incubation
• However, the rate of MUNANA substrate turnover remains linear for more than 2 hours with viral neuraminidase, allowing the assay to be performed for as little as 20 minutes to save time or for as long as 2 hours to increase signal output
• The assay can also be conducted in real-time without the addition of stop solution for those investigators who want to perform their own assay development or monitor rates of substrate turnover in the presence of inhibitor.