Molecular Probes™ LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation

Description

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation Kit has been optimized and validated for use with a UV laser flow cytometer Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs Optimal brightness The LIVE/DEAD™ Fixable Blue Stain was selected based on its fluorescent properties to provide a bright signal when excited with a UV laser The blue-fluorescent reactive dye has an excitation maximum of ∼350 nm, making it ideal for use with an UV laser, and an emission of ∼450 nm By switching viability staining from a channel off of the blue 488 nm laser to the UV laser, a channel is freed up on the flow cytometer for other reagents that are difficult to find in colors other than FITC or PE By using the UV laser, there is no spectral overlap issues with other common dyes, thus eliminating the need to compensate the viability stains from other stains in the panel Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments