Molecular Probes™ LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation

Description

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation Kit has been optimized and validated for use with a violet laser flow cytometer Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs Low compensation The LIVE/DEAD™ Fixable Violet Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser The violet-fluorescent reactive dye has an excitation maximum of ∼416 nm and is excited well with the 405 nm violet laser It has an emission maxima of ∼451 nm, so it can be collected in the first channel on most violet laser flow cytometers