7091870
Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)
A membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye.
Manufacturer: Fischer Scientific
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Color
Green
For Use With (Application)
Viability Assay
Product Type
Dye
Product Line
eBioscience™
Detection Method
Fluorescence
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Emission
495/515
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Description
- Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye Apoptotic and dead cells with compromised cell membranes do not retain Calcein Calcein is optimally excited at 495 nm and has a peak emission of 515 nm For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30) For multicolor flow cytometry, concentrations in the range of 5 to 20 nM are recommended to ensure that compensation can be performed successfully Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat
- 65-0855) or Calcein Violet 450 AM (cat
- 65-0854) Calcein AM should be reconstituted in high-quality, freshly opened DMSO Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time (avoid freeze-thawing) Purity: >95% (determined by HPLC)