7091871
Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514
Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.
Manufacturer: Fischer Scientific
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Detection Method
Fluorescence
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Emission
490⁄514
For Use With (Application)
Calcium Assay
Product Type
Dye
Product Line
eBioscience™
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Description
- Calcium binding affinity: Kd = 232 nM Molecular Weight: ≈1100 d Peak Excitation: 490nm Peak Emission: 514nm Reconstitute in high-quality, freshly opened DMSO Recommended concentration: 2 to 5 mM Once reconstituted, protect from light and store dessicated at −20°C; avoid freeze-thawing