Thermo Scientific™ beta-Galactosidase Assay Stop Solution

Description

• The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity
• The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity
• Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution
• Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity
• With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles
• The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification
• Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution): Choose stand-alone assay reagent or complete kit for cell lysis and detection Colorimetric assay easily measured in a plate reader or spectrophotometer (405 nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis Also compatiible with prepared lysates from other species such as bacteria Related Products Mammalian beta-Galactosidase Assay Kit (Cat
• No
• 75707) beta-Galactosidase Assay Reagent (Cat
• No
• 75705)