PI75768
Thermo Scientific™ Yeast beta-Galactosidase Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI75768-Each-of-1 | In Stock | ₹ 27,379.20 |
PI75768 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,379.20
GST (18%): ₹ 4,928.256
Total Price: ₹ 32,307.456
Content And Storage
Sufficient For: 250 colonies or 250 culture samples (100 μL each)Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
For Use With (Application)
Absorbance
Product Type
Yeast beta-Galactosidase Assay Kit
Format
96-well Plate
Quantity
75 mL
Substrate Type
Beta-Gal Substrate
Target
Beta-Galactosidase
Detection Method
Reporter Enzyme
For Use With (Equipment)
Microplate Reader
Dye Type
Enzyme Labeled
Product Line
Pierce™
Substrate
ONPG
Sufficient For
250 colonies or 250 culture samples (100 μL each)
Related Products
Description
- Thermo Scientific™ Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems
- The Yeast β-Galactosidase Assay Kit allows for qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium
- A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER™ Yeast Protein Extraction Reagent and the β-Galactosidase Assay Buffer
- After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution
- The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm
- Highlights: Efficient lysis of yeast cells and a colorimetric detection system Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis Also compatible with prepared lysates from other species such as bacteria Includes: Thermo Scientific Y-PER Yeast Protein Extraction Reagent (Part No
- 78990), 2X Assay Buffer and Stop Solution