K1514
Thermo Scientific™ CellSensor™ SBE-blaA375 Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1514-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1514 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
CellSensor™ SBE-bla A375 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Product Line
CellSensor™
Target Entry
ALK5, ALK7, TGF beta/BMP pathway
System Type
CellSensor™
Cell State
Dividing Cells
Detection Method
Fluorescence
Quantity
1 Vial
Cell Line
A375
Druggable Target
Signaling Pathway
Related Products
Description
- Bone morphogenetic proteins (BMPs), members of the transforming growth factor beta (TGF-β) superfamily, play important roles in the development of the heart, central nervous system and cartilage
- Disruption of BMP signaling affects the body plan of the developing embryo
- BMPs propagate their signal by activating Activin receptor-like kinase (ALK), which in turn mediates the phosphorylation of R-Smads
- Phosphorylated R-Smad associates with Smad4 and then translocates to the nucleus regulating gene expression
- A375 cells are human malignant melanoma cells containing endogenous Smad signaling pathway
- SBE-bla A375 cell line was engineered to express beta-lactamase under the control of Smad binding element
- This cell line has been validated for Z' and EC50 under optimized conditions using BMP-4
- This cell line has also responded to Nodal and was tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, substrate loading time
- Additional information using various small molecule inhibitors and StealthTM RNAi is also provided.