Invitrogen™ Exosome-Human CD63 Isolation/Detection Reagent (from cell culture media)

Description

• Exosome-Human CD63 Isolation/Detection Reagent enables exosome (also called extracellular vesicles and multi-vesicular bodies) detection using techniques such as flow cytometry, electron microscopy, or Western blotting
• The exosomes must be pre-enriched before isolation
• This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent
• Obtain highly pure CD63+ exosomes View sample during handling Easily scalable protocol Detect exosomes via flow cytometry, in less than 1 hour Utilizes Dynabeads magnetic separation technology Allows easy purification of pre-enriched CD63+ exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting Bead-bound exosomes can be easily visualized by flow cytometry Monodispersed and relatively large Dynabeads (4.5 μm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour Magnetic handling also allows sample to be visualized due to the light brown color of beads Magnets allow for easy separation and purification Sample and bead volume can be easily scaled up or down according to your sample size or downstream applications Good Mixing is Critical For successful exosome isolation, it is important to use a mixer that tilts and rotates to ensure that beads do not settle in tube Avoid end-over end rotation for small sample volumes (e.g., 100 μL) Please see user manual below for more guidelines