Invitrogen™ Exosome-Streptavidin Isolation/Detection Reagent

Description

• Exosome-Streptavidin Isolation/Detection Reagent, when combined with your choice of biotinylated primary antibody, enables the purification of exosomes (also called extracellular vesicles and multi-vesicular bodies) that can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting
• The exosomes must be pre-enriched before isolation
• This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent
• Maximum flexibility with your choice of biotinylated antibody See your sample during handling Easily scalable protocol Detect exosomes via flow cytometry, in less than 1 hour Utilizes Dynabeads magnetic separation technology Allows for easy purification of pre-enriched exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting Monodispersed and relatively large Dynabeads (4.5 μm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour Magnetic handling also allows sample to be visualized due to the light brown color of beads Good Mixing is Critical For successful exosome isolatio,n it is important to use a mixer that tilts and rotates to ensure that beads do not settle in tube Avoid end-over end rotation for small sample volumes (e.g., 100 μL) Please see user manual below for more guidelines