11-363-D
Invitrogen™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-363-D-Each-of-1 | In Stock | ₹ 1,02,158.64 |
11-363-D - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,02,158.64
GST (18%): ₹ 18,388.555
Total Price: ₹ 1,20,547.195
Cell Type
T Cells (Regulatory)
Product Type
Cell Isolation Kit
No. of Cells
Processes ∼1 x 109cells total
Product Line
DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
Quantity
2 x 5 mL
Shipping Condition
Room Temperature
Target Species
Human
Content And Storage
Each Kit Contains:• 2 mL Antibody Mix Human CD4• 10 mL Depletion MyOne™ Dynabeads™• 5 mL Dynabeads™ CD25• 2 mL DETACHaBEAD™.These reagents are sufficient to process ∼1 x 109cells. Store components at 2°C to 8°C.
Isolation Technology
Depletion, then positive isolation
Output Viability
>95%
Purity or Quality Grade
Research Grade
Sample Type
PBMC
Starting Material Cell No.
1 x 108PBMCs per isolation
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Description
- Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3
- The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays
- You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression
- Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells • Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain Foxp3+
- • Treg cells can be used in any downstream applications (e.g., suppression assays) Methodology The isolation procedure includes the following steps: • Negative isolation of untouched human CD4+ T-cells from PBMC
- • Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset
- • Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™ Starting sample PBMC Order Info Shipping Condition: Room Temperature