Description

• General description: Pyridoxal 5′-phosphate (PLP), an active form of vitamin B6/ pyridoxine is the coenzyme of amino acid metabolism.[1]
• Application: Pyridoxal 5′-phosphate monohydrate has been used:as a component in the reaction mixture for ornithine decarboxylase (ODC) activity assay[2] as a standard to quantify the concentration of pyridoxal 5′-phosphate (PLP) in cerebrospinal fluid (CSF) of children[3] as a dietary supplement to study its effects on the lethal phenotype of mutant flies[4]
• Biochem/physiol Actions: Pyridoxine participates in the production of lipid and neurotransmitters.[1] Pyridoxal 5′-phosphate (PLP) serves as a cofactor to stabilize carbanions at Cα of amino acids.[5] Pyridoxine deficiency results in peripheral neuropathy.[1] Absence of PLP leads to neonatal epileptic encephalopathy.[3] PLP acts as a coenzyme in transamination, decarboxylation and deamination reactions[6] such as the conversion of dopa into dopamine; of glutamate into γ aminobutyric acid (GABA); of histidine to histamine. PLP works mechanistically through formation of a Schiff-base.
• Other Notes: Coenzymes and Cofactors, vol. 1: Vitamin B6, Pyridoxal Phosphate[7][8]