Description

• General description: CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.[1]
• Application: Catalase from bovine liver is used for the following applications:Gel filtration[2]Analytical filtration on Ultrogel and glycerol gradient centrifugation[3]Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor[4]Enzyme assays[5][6]Alcohol oxidase activity[7]
• Biochem/physiol Actions: Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.
• Caution: Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.
• Unit Definition: One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
• Preparation Note: This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.