P7890

Poly-L-lysine hydrobromide

mol wt 15,000-30,000 by viscosity

Manufacturer: Sigma Aldrich

CAS Number: 25988-63-0

Synonym(S): L-Lysine homopolymer hydrobromide

Select a Size

Pack Size SKU Availability Price
25 MG P7890-25-MG In Stock ₹ 6,216.00
100 MG P7890-100-MG In Stock ₹ 16,561.20
500 MG P7890-500-MG In Stock ₹ 41,802.60
1 G P7890-1-G In Stock ₹ 69,408.30

P7890 - 25 MG

₹ 6,216.00

In Stock

Quantity

1

Base Price: ₹ 6,216.00

GST (18%): ₹ 1,118.88

Total Price: ₹ 7,334.88

form

powder

Quality Level

200

mol wt

15,000-30,000 by viscosity

color

white to off-white

application(s)

cell analysis

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

Other Options

Image Product Name Manufacturer Price Range
Electron Microscopy Sciences Poly-L-Lysine 0.1% Solution, 25988-63-0, 10ml
-- ₹ 3,422.40
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine Solution | 25988-63-0 | MFCD00165424 | 500ml
-- ₹ 55,340.21
Sigma Aldrich Fine Chemicals Biosciences Poly-L-lysine solution | 25988-63-0 | MFCD00165424 | 100 mL
-- ₹ 16,455.75
Supply Solutions Poly-L-lysine solution, 25988-63-0, MFCD00165424, 50mL
-- ₹ 12,423.31
Poly-L-lysine hydrobromide
-- ₹ 6,490.00 - ₹ 1,02,641.70
Poly-L-lysine hydrobromide
-- ₹ 7,040.00 - ₹ 77,011.80
Poly-L-lysine hydrobromide
-- ₹ 7,004.10 - ₹ 68,609.10
Poly-L-lysine hydrobromide
-- ₹ 4,972.80 - ₹ 67,743.30
Poly-L-lysine hydrobromide
-- ₹ 7,703.40 - ₹ 72,405.30
Poly-L-lysine hydrobromide
-- ₹ 5,183.70 - ₹ 68,331.60

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Description

  • Application: Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.
  • Biochem/physiol Actions: Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.
  • Components: Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
  • Caution: Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
  • Preparation Note: This product has a molecular weight of 15,000-30,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
  • Analysis Note: Molecular weight based on viscosity and was also assayed by MALLS.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves