Description

• General description: 3,3′,5′-Triiodo-L-thyronine (T3) is an endocrine hormone produced by the thyroid gland and is regarded as an active thyroid hormone at cellular level.[1][2] Thyroxine (T4) serves as a precursor for T3 and is generated in extrathyroidal tissues.[3]
• Application: 3,3′,5-Triiodo-L-thyronine sodium salt has been used for inducing thyroid hormone manipulations in mice.[4] It has also been used In cell culturing of hippocampal,[5] retinal,[6] proximal tubule and glomerular explant.[7]
• Biochem/physiol Actions: 3,3′,5′-Triiodo-L-thyronine (T3) increases the rates of protein synthesis, stimulates the breakdown of cholesterol and effects embryonic development. In cell culture, T3 regulates cell differentiation and protein expression. The interaction of T3 with nonhistone proteins in the chromatin initiates the thyroid hormone activity. Thyroid hormones regulate the differentiation and development of tissues and affect a number of metabolic processes.[8] The level of circulating T3 hormone is reduced in hypothyroidism.[1]
• Physical form: powder-20 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw, solution stable 2-8 °C, 30 days
• Reconstitution: To prepare 20 μg/ml stock solution: add 1ml 1N NaOH per mg 3,3′, 5-triiodo-L-thyronine; gently swirl to dissolve. To this, add 49 ml sterile medium per ml 1N NaOH added.