Gibco™ CO2Independent Medium

Description

• CO 2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO 2 incubator CO 2 Independent Medium is ideal for transporting cells or tissue, and for the handling of mouse embryos under atmospheric conditions Gibco™ CO 2 Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems Additionally, Gibco™ CO 2 Independent Medium has been formulated with components that enhance cellular production and utilization of CO 2 Store at 2°C to 8°C, protected from light Modified as follows With: phenol red, sodium pyruvate Without: L-glutamine, HEPES Using CO 2 Independent Medium CO 2 Independent Medium contains no lipids or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS) The CO 2 Independent Medium does not require a CO 2 environment to maintain physiological pH For maximum growth performance some cell lines may require either direct or sequential adaptation to CO 2 Independent Medium