Description

• General description: D(+)-Biotin is the simplest reagent for the introduction of biotin to peptides, proteins and other biomolecules. In SPPS, it is best coupled using DIPCDI/Oxyma Pure. Biotin-labeled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.[1],[2],[3],[4]Associated Protocols and Technical ArticlesBiotinylation Reagents for Peptide SynthesisLiterature references:[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516. [2] T. Buranda, et al. (1999) Cytometry, 37, 21.[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127. [4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.
• Application: Some recent applications of D(+)-Biotin in SPPS:In the high-throughput biotinylation of proteins. The approach for site-specific biotinylation of proteins is useful for various applications such as protein purification and analysis.[5]In the site-specific biotinylation of multipodal glycoclusters on a solid support. The synthesis of biotinylated glycoclusters, has important applications in glycobiology.[6]
• Legal Information: Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany