Description

• General description: Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.
• Application: Trizma has been used:In the dissection medium and culture medium of Hippocampal organotypic tissue cultures[3]As a component of lysis buffer before immunoprecipitation and immunoblotting[4]As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding[5]As a component of mixed buffer formulations[6][7]As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting[7]
• Other Notes: Easily compare specifications for Trizma products with the Trizma specification table.
• Legal Information: Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany