P3563
Phosphate Buffered Saline
powder, pH 7.4, with 0.05% Tween® 20
Manufacturer: Sigma Aldrich
Synonym(S): 1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 PKG | P3563-10-PKG | In Stock | ₹ 6,249.30 |
| 5 X 10 PKG | P3563-5-X-10-PKG | In Stock | ₹ 24,808.50 |
P3563 - 10 PKG
In Stock
Quantity
1
Base Price: ₹ 6,249.30
GST (18%): ₹ 1,124.874
Total Price: ₹ 7,374.174
product name
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,
form
powder
Quality Level
200
solubility
water: soluble, clear, colorless (1 pouch dissolved in 1L)
application(s)
cell analysisdiagnostic assay manufacturing
Related Products
Description
- General description: Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.
- Application: Phosphate buffered saline has been used in:western blottingenzyme-linked immunosorbent assay (ELISA)[1]telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)[2][3][4]cell isolation[5]
- Packaging: Foil pouches
- Reconstitution: Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.
- Other Notes: For additional information on our range of Biochemicals, please complete this form.
- Legal Information: TWEEN is a registered trademark of Croda International PLC
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves