75633

Osmium tetroxide solution

suitable for electron microscopy, 2% in H2O

Manufacturer: Sigma Aldrich

CAS Number: 20816-12-0

Synonym(S): Osmium(IV) oxide

Select a Size

Pack Size SKU Availability Price
2 ML 75633-2-ML In Stock ₹ 20,102.10

75633 - 2 ML

₹ 20,102.10

In Stock

Quantity

1

Base Price: ₹ 20,102.10

GST (18%): ₹ 3,618.378

Total Price: ₹ 23,720.478

form

solution

Quality Level

100

reaction suitability

reagent type: oxidant

concentration

2% in H2O

technique(s)

electron microscopy: suitable

color

colorless to yellow

density

1.0 g/mL at 20 °C1.0 g/mL at 20 °C

application(s)

hematologyhistology

storage temp.

2-8°C

SMILES string

O=[Os](=O)(=O)=O

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Description

  • General description: Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.
  • Application: Osmium tetroxide solution has been used as a fixative in electron microscopy.[1][2]
  • Biochem/physiol Actions: Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.[3]

SAFETY INFORMATION

Pictograms

GHS06,GHS05

Signal Word

Danger

Hazard Statements

H302 + H332,H310,H315,H318

Precautionary Statements

P262 - P280 - P301 + P312 - P302 + P352 + P310 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves