S05651
Edvotek™ Purification and Size Determination of Green and Blue Fluorescent Proteins
Manufacturer: Edvotek™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | S05651-Each-of-1 | In Stock | ₹ 11,636.16 |
S05651 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 11,636.16
GST (18%): ₹ 2,094.509
Total Price: ₹ 13,730.669
Product Type
Green and Blue Fluorescent Protein Kit
Includes
Instructions, columns and matrix, gfp and bfp extracts, buffer, protein gel reagents for optional activity
Content And Storage
Refrigerated. Some components require freezer storage.
For Use With (Application)
Used as an assay during their purification from an e. coli extract
Time Range
4 hours 15 minutes; Staining time: 30 minutes
Class Size
6 Lab Groups
Related Products
Description
- When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins
- Unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E
- coli extract Column fractions containing GFP or BFP are identified by fluorescence and then purified As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis to estimate the purity and size of the GFP and BFP proteins Experiment times: Packing and running column, 45 minutes Optional electrophoresis, one hour Staining, 30 minutes Destaining, two hours All You Need water bath long wave UV lamp ring stand; clamps automatic micropipet vertical gel electrophoresis apparatus power supply hot plate polyacrylamide gels (12%) Order Info For six lab groups
- Recommended Storage Some components require freezer storage