S05656
Edvotek™ Purification of the Restriction Enzyme Eco RI
Manufacturer: Edvotek™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | S05656-Each-of-1 | In Stock | ₹ 27,978.12 |
S05656 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,978.12
GST (18%): ₹ 5,036.062
Total Price: ₹ 33,014.182
Product Type
Purification Kit
Includes
Instructions, ion exchange matrix, chromatography columns, E. coli cell extract, equilibration and elution Buffer, Lambda DNA, Lambda or Eco RI Marker, KCl, glycerol, dilution and reaction Buffers, gel loading solution, Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide
Time Range
3 hours 15 minutes
For Use With (Application)
Purify the restriction enzyme, eco ri
Quantity
5 purifications
Content And Storage
Refrigerated. Some components require freezer storage.
Related Products
Description
- In this experiment, students actually purify the restriction enzyme, Eco RI
- This procedure utilizes an ion exchange chromatography step for Eco RI purification
- Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis Fractions that contain Eco RI are identified and pooled Total and specific activities are calculated Recommended for college level courses Experiment times: Packing column, 45 minutes Restriction analysis A, 35 minutes Restriction analysis B, 50 minutes Gel Prep, 30 minutes Electrophoresis, 30 minutes Staining and destaining, two minutes All You Need horizontal gel electrophoresis apparatus power supply UV visualization system waterbath microcentrifuge microwave or hot plate UV spectrophotometer & cuvettes automatic micropipet with tips ring stands & clamps 10 ml pipets lab glassware ice and ice buckets
- Order Info For five purifications Recommended Storage Some components requre freezer storage