15-224-041

Invitrogen™ T4 DNA Ligase (5 U/μL)

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 15-224-041-Each-of-1 In Stock ₹ 17,368.68

15-224-041 - Each of 1

₹ 17,368.68

In Stock

Quantity

1

Base Price: ₹ 17,368.68

GST (18%): ₹ 3,126.362

Total Price: ₹ 20,495.042

Concentration

5 U/μL

Shipping Condition

Dry Ice

Compatible Buffer

5X Reaction Buffer

Product Type

T4 DNA Ligase

Content And Storage

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

Enzyme

Ligase

Quantity

250 U

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Description

  • The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes
  • Single-stranded nucleic acids are not substrates for this enzyme
  • Unit Definition: One unit catalyzes the exchange of 1nmol 32 P-labeled pyrophosphate into ATP in 20 min
  • at 37°C
  • (One unit is equal to approximately 300 cohesive-end ligation units.) Unit Reaction Conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl 2 , 10mM DTT, 66μM ATP, 3.3μM 32 P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min
  • at 37°C
  • Order Info Shipping Condition: Dry Ice