15-224-090
Invitrogen™ T4 DNA Ligase (1 U/μL)
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 15-224-090-Each-of-1 | In Stock | ₹ 94,287.12 |
15-224-090 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 94,287.12
GST (18%): ₹ 16,971.682
Total Price: ₹ 1,11,258.802
Concentration
1 U/μL
Enzyme
Ligase
Quantity
4 x 500 U
Content And Storage
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.
Compatible Buffer
5X Reaction Buffer
Product Type
T4 DNA Ligase
Related Products
Description
- T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini
- Single-stranded nucleic acids are not substrates for this enzyme
- Optimizes ligation in 5 minutes Source: Purified from E
- coli lambda lysogen NM989 Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested Unit definition: One unit catalyzes exchange of 1nmol 32 P-labeled pyrophosphate into ATP in 20 min
- at 37°C
- One unit is equal to approximately 300 cohesive-end ligation units Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl 2 , 10mM DTT, 66μM ATP, 3.3μM 32 P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min
- at 37°C ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning Order Info Shipping Conditions: Dry ice