3509028

Promega SP6 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Manufacturer: Fischer Scientific

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Color

Colorless

Purity

>90%

For Use With (Application)

Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA

Content And Storage

-30°C to -10°C

Product Type

SP6 RNA Polymerase

Components

100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl

pH

7.5

Storage Buffer

20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol

Source

Recombinant E. coli strain

Form

Liquid

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Description

  • DNA-Dependent RNA Polymerase Specific for SP6 Promoter Sequences Exhibits extremely high affinity and specificity for SP6 promoter sequences >90% pure as determined by SDS polyacrylamide gel electrophoresis Free of detectable levels of contaminating RNase and DNase activity (